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Human Primary Prostate Stromal Mesenchymal Stem Cells (HPS-19I)

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SKU: T4199 Category: Tag:

Description

key features

Cat.No.:T4199

Quantity:5 x 105 cells/1.0ml

 

Organism Homo sapiens
Source Organ Prostate Gland
Donor Gender Male
BioSafety Level II
Growth Properties Adherent
Morphology Spindle-shape fibroblast-like
Disease Normal
Description Mesenchymal stem cells are multipotent stromal cells with the ability to differentiate into other cell lineages such as cartilage, bone, and adipose tissues. Tissue-resident mesenchymal stem cells may have a role in repair, fibrosis, cardiovascular diseases, and tumor microenvironment. Studies have suggested that formation of myofibroblasts is associated with diseases such as cancer, fibrosis, and vessel disease. The Human Prostate Stromal Mesenchymal Stem Cells (HPS-19I) are isolated CD44+CD90+ cells from the adult prostate gland which has the ability to differentiate into neurogenic, chondrogenic, and osteogenic cell lineages. In the presence of TGF-β1 the cells differentiate into myofibroblasts by inhibiting RUNX1 expression. The cells potentially are a source of reactive stroma fibroblasts. These cells are recommended in use for stem cell biology, stroma microenvironment, anticancer therapeutic and antifibrotic therapeutic studies.
Applications For Research Use Only
Markers CD44, CD90, CD13, CD29, CD73, CD105
Image
Propagation Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow III medium available from ABM (TM003). To make the completed growth medium, add the following components to the base medium to a final concentration of: 5% fetal bovine serum (TM999), 5% NuSerum, 0.5 µg/ml testosterone, 5 µg/ml insulin, and Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO₂: 5%; Temperature: 37.0°C.

Note: Density-dependent inhibition is exhibited at more than 70% confluency.

Procedure Overview
Quality Control Flow cytometric analysis was performed to determine surface markers

Important Considerations For Primary Cells

Primary Cell Handling Instructions Upon Arrival

Subculturing Protocol

Freezing Protocol

Thawing Protocol