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Human Primary Intestinal Smooth Muscle Cells


SKU: T4211 Category: Tag:


key features


Quantity:5 x 105 cells/1.0ml

Organism Homo sapiens
Source Organ Intestine
BioSafety Level II
Growth Properties Adherent
Morphology Multipolar
Recommended Seeding Density 6,000-10,000 cells/cm2
Disease Normal
Applications For Research Use Only
Markers alpha-actin
Propagation Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow X series medium available in ABM, Cat. No. TM4211. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 10% and Penicillin/Streptomycin(G255). Atmosphere: air, 95%; Carbon dioxide (CO2), 5%.
Procedure Overview

Important Considerations For Primary Cells

Primary Cell Handling Instructions Upon Arrival

Subculturing Protocol

Freezing Protocol

Thawing Protocol