4 well silicone insert with 4 defined cell-free gaps, suitable for wound healing, migration assays, 2D invasion assays, and co-cultivation of cells
Complete solution for wound healing experiments, requiring only a few steps from sample preparation to image analysis
Reproducible experiments owing to: four defined 500 µm cell free gaps and one 1000 µm center gap, no leakage during cultivation, and no material being left behind after the insert’s removal
Suitable for 4 experiments, including a center gap that allows to observe the http://www.atlantisbioscience.com.sg/home/wp-content/uploads/2019/02/IN_811XX_Dish35mm_high-5.pdfmigration of all cell types
Clean surface after insert removal without remaining material
Find full specifications and technical details of the µ-Dish here
Principle for Wound Healing and Migration Assays
Wound Healing / Migration
The Culture-Insert 4 Well allows for wound healing and migration assays with four cell-free gaps with a 0.5 mm gap width, and one center area with a 1 mm diagonal distance. Compared to the 2 well version, more cells can be analyzed and a higher throughput is realized. Two cell-free gaps create a technical replicate in the very same well.
Two Conditions of Wound Healing / Migration
With the Culture-Insert 4 Well, a wound healing experiment and a control experiment can be done in the same well. The control cells can be pre-treated with any kind of treatment (e.g., drugs, plasmids, mRNA, or virus particles).
Four Conditions for Co-Cultivation / Invasion
By seeding up to four different cell types, the invasiveness of adherent cells can be analyzed. Non-invasive cell types can be cultured next to each other, sharing the same medium. This co-culture allows the cells to communicate and share factors for differentiation studies or long-term culturing.
Invasion and co-cultivated cells can be imaged by using high-resolution fluorescence microscopy.