Generation of safe iPSC is critical for medical processes. To achieve that, a feeder free culture system must be designed under chemically defined conditions. Such a system must allow mass cultures of iPSCs.
Currently , feeder cells are needed for culture of ES cells and iPSCs. Matrigel is widely used when a researcher wants a feeder free culture system.
However Matrigel have several unknown components creating batch inconsistency issues.
We are pleased to introduce iMatrix-511 a suitable substrate for iPSC and ESC culture
1. Drastically shorten preparation time using iMatrix 511 silk versus conventional methods
Conventionally, feeder cells take a long time to prepare as it requires precise and controlled dissociation. With iMatrix 511 silk , time is drastically saved.
2. Ease of Handling
iMatrix 511 silk is easy to use
3. Greater efficiency in bulk proliferation over conventional methods
Traditionally, complete dissociation of iPSC colonies lead to extensive cell death . Large aggregates results in spontaneous differentiation. With iMatrix 511 silk complete dissociation of iPSC is possible without extensive cell death.
4. Higher Passage Ratio
With iMatrix 511 silk a higher passaging ratio is possible during subculture which was approximately 1: 10 compared with 1:4 for conventional methid.
5. Safety and high quality of substrate
Traditional feeder cells are derived from mouse and the popular matrigel is a substrate derived from EHS mouse sarcoma cells; giving rise to safety issues for medical applications.
iMatrix 511 silk
Highly purified by chromatography and processed in cGMP banked cells. Risk of xenogeneic contamination is extremely low.
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