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µ-Slide I Luer

$1.00

Description

Channel slides with different heights, volumes, and coatings specially suited for flow applications

key features

  • Large area of uniform shear stress
  • Easy connection using Luer adapters
  • Homogeneous cell distribution with defined optical pathway

Instruction menu

Instruction menu

Applications

  • Adherent cells under flow conditions
  • Cell culture (static or stop-flow)
  • 3D cell culture in gels brought into the channels
  • High-resolution microscopy of living and fixed cells

Specifications

Channel length 50 mm
Channel width 5 mm
Adapters Female Luer
Volume per reservoir 60 μl
Growth area 2.5 cm2
Coating area 5.2 / 5.4 / 5.6 / 5.8 cm2
Bottom: ibidi Polymer Coverslip

Technical Features

  • Standard format with thin bottom for low or high magnification microscopy (up to 100x)
  • Large observation area for microscopy
  • Channel volumes of 50 μl, 100 μl, 150 μl, or 200 μl
  • Defined shear stress and shear rate levels
  • Easy connection to tubes and pumps using Luer adapters
  • Available as variety pack containing all heights
  • Fully compatible with the ibidi Pump System

Cells Cultured Under Static or Flow Conditions

Flow cultivation

Static cultivation

Cross Section of the Channel: Same Slide – Different Channel Height and Volume

* not recommended for static culture over more than 6 hours

Choosing the Right Channel for Flow Applications

For flow assays with small amounts of
medium and high values of shear stress
0.2 mm channel
For a wide range of shear stress 0.4 mm channel
For controlling low values of shear stress
(<2 dyne /cm²)
0.6 and 0.8 mm
channels

Static Cultures vs. Flow Applications

The general rule is:
Low channels are more suitable for flow applications.
High channels are more suitable for static cell culture.

Select the ideal Perfusion Set for your flow application here.

Compatible with Solvents for Staining and Fixation

HUVEC, cultivated over 7 days at 10 dyn / cm². VE-cadherins are stained in green, cell nuclei are stained in blue.

Influence of Shear Stress on Cultured Cells

Human umbilical vein endothelial cells (HUVEC) cultured under flow conditions (20 dyn / cm²) in a µ-Slide I 0.4 Luer over 9 days. The primary cells were transduced with the adenoviral vector rAV CMV-LifeAct-TagRFP 24 hours prior to the experiment.