- Cultivation and microscopy of cell cultures
- Transfection assays
- Fluorescence microscopy of both living and fixed cells
- Live cell imaging over extended time periods
Phase contrast microscopy is the most commonly used, transmitted light technique in cell culture. When working with phase contrast microscopy, it is crucial to have the two phase rings adjusted to each other. In open wells, the meniscus at the air-water-interphase works like a lens that refracts the beam path. This miscalibrates the phase rings, leading to poor contrast in the microscopic image.
Working with the µ-Slide 4 Well Ph+ diminishes the meniscus, so that the whole optical system is aligned, no matter which position of the well is being imaged.