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µ-Dish 50 mm, low

$1.00

SKU: 81136 Category: Tag:

Description

A 50 mm imaging dish with an ibidi Standard Bottom and low walls, used for high end microscopy and micromanipulation

key features

  • Large access for micromanipulation
  • Unique ibidi Polymer Coverslip combining
    • Excellent cell culture conditions; and
    • Supreme optical quality for high resolution microscopy
  • Ideal cell growth conditions provided by the ibiTreat surface
  • Lid with lock position, which minimizes evaporation
  • Suitable for DIC, when used with the special DIC Lid
  • Also available with a grid

Instruction menu

Instruction menu
Applications
  • Cell culture and high resolution fluorescence microscopy
  • Cultivation of a maximum number of cells
  • Micromanipulation of single cells
  • Fluorescence microscopy of both living and fixed cells
Specifications
Ø µ-Dish 50 mm
Volume 3 ml
Growth area 7.0 cm2
Coating area using 400 µl 7.9 cm2
Ø observation area 30 mm
Height with / without lid 12 / 9 mm
Bottom: ibidi Polymer Coverslip
Technical Features
  • Standard format dish with a 50 mm diameter
  • Made of biocompatible material using no glues, consequently no cells are harmed
  • Rim for easy opening
  • No autofluorescence
  • Compatible with all staining and fixation solvents


Low walls
 – Large access for micromanipulation

Experimental Example

Immunofluorescence of Rat1 fibroblast cells. The F-actin cytoskeleton is stained with Phalloidin (Alexa488, green), the nucleus is stained with DAPI (blue). Widefield fluorescence with 100x objective lens.

Lid with Locking Feature for Minimized Evaporation

All ibidi µ-Dishes are equipped with the special lid locking feature. The locking position minimizes evaporation and thereby provides excellent conditions for long-term studies in a non-humidified environment. Gas exchange (carbon dioxide, or oxygen) during cell culture is maintained thanks to the gas-permeable plastic material of the dish.

TIP: Use the locking feature only if minimal evaporation is required (e.g., outside incubators, non-humidified microscopy stages, etc.).

ibidi µ-Dish Family: Versions and Variations

High walls: 
Used for all standard applications

Low walls:
Provide greater cell access (useful for micromanipulation)

ibidi Polymer Coverslip vs. ibidi Glass Bottom

ibidi Polymer Coverslip

ibidi Glass Bottom

Optical properties
Refractive index (nD 589 nm) 1.52 1.52
Abbe number 56 55
Thickness #1.5 (180 µm) #1.5H (170 µm)
Material Microscopy plastic D 263M Schott borosilicate glass
Autofluorescence Low Low
Transmission Very high (even ultraviolet) High (ultraviolet restrictions)
Birefringence (DIC) Low (DIC compatible*) Low (DIC compatible*)
Other aspects
Surface modifications ibiTreat – tissue culture treated
Uncoated – hydrophobic
Only glass
Protein coatings Compatible Compatible
Gas permeable Yes No
Material flexibility High Low
Breakable No Yes
Applications Fluorescence microscopy TIRF and single photon

* Special glass DIC Lids are available separately